BolC9t58100H-R-P (polypeptide) Brassica oleracea HDEM

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview


Name	BolC9t58100H-R
Unique Name	BolC9t58100H-R-P
Type	polypeptide
Organism	Brassica oleracea HDEM (cv. HDEM, Wild cabbage)
Sequence length	199

Homology

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BLAST of BolC9t58100H-R vs. GenBank_protein
Match: VDD32777.1 (unnamed protein product [Brassica oleracea])

HSP 1 Score: 383 bits (983), Expect = 7.660e-134
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query:    1 MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLKTSSLWRDHVQTYT 198
            MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLKTSSLWRDHVQTYT
Sbjct:    1 MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLKTSSLWRDHVQTYT 198

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BLAST of BolC9t58100H-R vs. GenBank_protein
Match: CAF1771111.1 (unnamed protein product [Brassica napus])

HSP 1 Score: 353 bits (906), Expect = 4.280e-120
Identity = 184/185 (99.46%), Postives = 184/185 (99.46%), Query Frame = 0

Query:    1 MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLK 185
            MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDV K
Sbjct:    1 MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVHK 185

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BLAST of BolC9t58100H-R vs. GenBank_protein
Match: CDY66234.1 (BnaCnng50070D [Brassica napus])

HSP 1 Score: 307 bits (786), Expect = 7.550e-104
Identity = 171/198 (86.36%), Postives = 174/198 (87.88%), Query Frame = 0

Query:    1 MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLKTSSLWRDHVQTYT 198
            M SP TC+VFHLKRMIETHHNRRLSRSSRRLV WFSSS TYDDSIS HGVSSSGGRCLQFGDQLL      SKHMLLRQL   DSNEESSLSSS DMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRR        +EEPLTVKV+HLKDKPFDMDDDVIKATSFE ISTLRDVLKTSSLWRDHVQTYT
Sbjct:    7 MVSPLTCLVFHLKRMIETHHNRRLSRSSRRLVCWFSSSPTYDDSISPHGVSSSGGRCLQFGDQLL----DLSKHMLLRQL---DSNEESSLSSSADMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRLRITEM-VSEEPLTVKVDHLKDKPFDMDDDVIKATSFEVISTLRDVLKTSSLWRDHVQTYT 196

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BLAST of BolC9t58100H-R vs. GenBank_protein
Match: CDY33341.1 (BnaC05g12420D [Brassica napus])

HSP 1 Score: 292 bits (748), Expect = 5.690e-98
Identity = 161/185 (87.03%), Postives = 165/185 (89.19%), Query Frame = 0

Query:    1 MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLK 185
            MASPPTC+VFHLKRMI+THHNRRLSRSSRRLVRWFSSS TYDDSIS HGVSSSGGRCLQFGDQLL      SKHML RQLKQLDSNEESSLSSS DMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRR        +EEPLTVKV+HLKDKPFDMDDDVIKATSFE ISTLRDV K
Sbjct:    1 MASPPTCLVFHLKRMIKTHHNRRLSRSSRRLVRWFSSSPTYDDSISPHGVSSSGGRCLQFGDQLL----DLSKHMLHRQLKQLDSNEESSLSSSADMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRLRITEK-VSEEPLTVKVDHLKDKPFDMDDDVIKATSFEVISTLRDVHK 180

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BLAST of BolC9t58100H-R vs. GenBank_protein
Match: VDD45106.1 (unnamed protein product [Brassica oleracea])

HSP 1 Score: 294 bits (752), Expect = 5.890e-98
Identity = 162/185 (87.57%), Postives = 165/185 (89.19%), Query Frame = 0

Query:    1 MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLK 185
            MASPPTC+VFHLKRMIETHHNRRLSRSSRRLVRWFSSS TYDDSIS HGVSSSGGRCLQFGDQLL      SKHML RQLKQLDSNEESSLSSS DMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRR        +EEPLTVKV+HLKDKPFDMDDDVIKATSFE ISTLRDV K
Sbjct:    1 MASPPTCLVFHLKRMIETHHNRRLSRSSRRLVRWFSSSPTYDDSISPHGVSSSGGRCLQFGDQLL----DLSKHMLHRQLKQLDSNEESSLSSSADMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRLRITEK-VSEEPLTVKVDHLKDKPFDMDDDVIKATSFEVISTLRDVHK 180

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BLAST of BolC9t58100H-R vs. GenBank_protein
Match: VDD53904.1 (unnamed protein product [Brassica oleracea])

HSP 1 Score: 268 bits (685), Expect = 1.470e-88
Identity = 154/196 (78.57%), Postives = 161/196 (82.14%), Query Frame = 0

Query:    3 SPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLKTSSLWRDHVQTYT 198
            SP TC+VFHLKRMIETHHNRRLSRSSRRLV WFSSS TYDDS+S HGVS SGGRCLQFGDQLL      SKHMLLRQLKQLDSNE       + +EK    L+   LLKRLHEVGTLAQISSIQGDQVILVGHRR        +EEPLTVKV+HLKDKPFDMDDDVIKATSFE ISTLRDVLKTSSLWRDHVQTYT
Sbjct:    9 SPLTCLVFHLKRMIETHHNRRLSRSSRRLVCWFSSSPTYDDSLSPHGVSPSGGRCLQFGDQLL----DLSKHMLLRQLKQLDSNE-------IMVEKRALFLQA--LLKRLHEVGTLAQISSIQGDQVILVGHRRLRITEM-VSEEPLTVKVDHLKDKPFDMDDDVIKATSFEVISTLRDVLKTSSLWRDHVQTYT 190

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BLAST of BolC9t58100H-R vs. GenBank_protein
Match: KAG2275996.1 (hypothetical protein Bca52824_058551 [Brassica carinata])

HSP 1 Score: 216 bits (551), Expect = 1.120e-67
Identity = 125/167 (74.85%), Postives = 132/167 (79.04%), Query Frame = 0

Query:   15 MIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGVSSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLR 181
            MI+THHNRRLSRSSRRLVRWFSSS TYDDSIS HGVSSSGGRCLQFGDQLL+  SS S H+            E++     DMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRR        +EEPLTVKV+HLKDKPFDMDDDVIKATSFE    LR
Sbjct:    1 MIKTHHNRRLSRSSRRLVRWFSSSPTYDDSISPHGVSSSGGRCLQFGDQLLIS-SSVSPHIPGPIKAYASPTAETARLQCADMEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRLRITEK-VSEEPLTVKVDHLKDKPFDMDDDVIKATSFEVHKRLR 165

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BLAST of BolC9t58100H-R vs. GenBank_protein
Match: XP_013729631.1 (lon protease homolog 1, mitochondrial-like [Brassica napus] >XP_022563461.1 lon protease homolog 1, mitochondrial-like [Brassica napus])

HSP 1 Score: 171 bits (432), Expect = 2.370e-51
Identity = 91/102 (89.22%), Postives = 93/102 (91.18%), Query Frame = 0

Query:   97 MEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLKTSSLWRDHVQTYT 198
            MEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRR        +EEPLTVKV+HLKDKPFDMDDDVIKATSFE ISTLRDVLKTSSLWRDHVQTYT
Sbjct:    1 MEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRLRITEK-VSEEPLTVKVDHLKDKPFDMDDDVIKATSFEVISTLRDVLKTSSLWRDHVQTYT 101

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BLAST of BolC9t58100H-R vs. GenBank_protein
Match: XP_013603955.1 (PREDICTED: lon protease homolog 1, mitochondrial-like [Brassica oleracea var. oleracea] >XP_013603956.1 PREDICTED: lon protease homolog 1, mitochondrial-like [Brassica oleracea var. oleracea])

HSP 1 Score: 170 bits (431), Expect = 3.370e-51
Identity = 91/102 (89.22%), Postives = 93/102 (91.18%), Query Frame = 0

Query:   97 MEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLKTSSLWRDHVQTYT 198
            MEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRR        +EEPLTVKV+HLKDKPFDMDDDVIKATSFE ISTLRDVLKTSSLWRDHVQTYT
Sbjct:    1 MEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRLRITEM-VSEEPLTVKVDHLKDKPFDMDDDVIKATSFEVISTLRDVLKTSSLWRDHVQTYT 101

[Close]

BLAST of BolC9t58100H-R vs. GenBank_protein
Match: XP_013585311.1 (PREDICTED: lon protease homolog 1, mitochondrial-like [Brassica oleracea var. oleracea])

HSP 1 Score: 169 bits (428), Expect = 9.610e-51
Identity = 90/102 (88.24%), Postives = 92/102 (90.20%), Query Frame = 0

Query:   97 MEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPLTVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLKTSSLWRDHVQTYT 198
            MEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRR        +EEPLT KV+HLKDKPFDMDDDVIKATSFE ISTLRDVLKTSSLWRDHVQTYT
Sbjct:    1 MEKNINDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRLRITEK-VSEEPLTAKVDHLKDKPFDMDDDVIKATSFEVISTLRDVLKTSSLWRDHVQTYT 101

The following BLAST results are available for this feature:

BLAST of BolC9t58100H-R vs. GenBank_protein

BLAST of BolC9t58100H-R vs. GenBank_protein
Analysis Date: 2022-03-19 (Diamond on OGS1.0 vs NR)
Total hits: 20


Match Name	E-value	Identity	Description
VDD32777.1	7.660e-134	100.00	unnamed protein product [Brassica oleracea]	[more]
CAF1771111.1	4.280e-120	99.46	unnamed protein product [Brassica napus]	[more]
CDY66234.1	7.550e-104	86.36	BnaCnng50070D [Brassica napus]	[more]
CDY33341.1	5.690e-98	87.03	BnaC05g12420D [Brassica napus]	[more]
VDD45106.1	5.890e-98	87.57	unnamed protein product [Brassica oleracea]	[more]
VDD53904.1	1.470e-88	78.57	unnamed protein product [Brassica oleracea]	[more]
KAG2275996.1	1.120e-67	74.85	hypothetical protein Bca52824_058551 [Brassica car...	[more]
XP_013729631.1	2.370e-51	89.22	lon protease homolog 1, mitochondrial-like [Brassi...	[more]
XP_013603955.1	3.370e-51	89.22	PREDICTED: lon protease homolog 1, mitochondrial-l...	[more]
XP_013585311.1	9.610e-51	88.24	PREDICTED: lon protease homolog 1, mitochondrial-l...	[more]

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GO Annotation

GO Assignments

This polypeptide is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034599	cellular response to oxidative stress
biological_process	GO:0051131	chaperone-mediated protein complex assembly
biological_process	GO:0007005	mitochondrion organization
biological_process	GO:0070407	oxidation-dependent protein catabolic process
biological_process	GO:0030163	protein catabolic process
biological_process	GO:0006515	protein quality control for misfolded or incompletely synthesized proteins
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004176	ATP-dependent peptidase activity
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0003697	single-stranded DNA binding

InterPro

Analysis Name: Interproscan on OGS1.0
Date Performed: 2022-03-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003111	Lon, substrate-binding domain	PFAM	PF02190	LON_substr_bdg	coord: 103..182 e-value: 6.8E-8 score: 32.6
IPR027065	Lon protease	PANTHER	PTHR43718	LON PROTEASE	coord: 88..198
None	No IPR available	PANTHER	PTHR43718:SF8	LON PROTEASE HOMOLOG 1, MITOCHONDRIAL-RELATED	coord: 88..198

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
C9	contig	C9:49680451..49681530 -

Analyses

This polypeptide is derived from or has results from the following analyses


Analysis Name	Date Performed
Interproscan on OGS1.0	2022-03-19
Blast2go on OGS1.0	2022-03-19
Diamond on OGS1.0 vs NR	2022-03-19
Brassica oleracea HDEM OGS1.0	2018-11-02

Relationships

This polypeptide derives from the following mRNA feature(s):


Feature Name	Unique Name	Species	Type	Position
BolC9t58100H-R	BolC9t58100H-R	Brassica oleracea HDEM	mRNA	C9 49680451..49681530 -

Sequences

The following sequences are available for this feature:

polypeptide sequence

polypeptide sequence

>BolC9t58100H-R-P ID=BolC9t58100H-R-P|Name=BolC9t58100H-R|organism=Brassica oleracea HDEM|type=polypeptide|length=199bp
MASPPTCIVFHLKRMIETHHNRRLSRSSRRLVRWFSSSSTYDDSISSHGV
SSSGGRCLQFGDQLLLCFSSYSKHMLLRQLKQLDSNEESSLSSSVDMEKN
INDLKGKDLLKRLHEVGTLAQISSIQGDQVILVGHRRTASHNGDGNEEPL
TVKVNHLKDKPFDMDDDVIKATSFEAISTLRDVLKTSSLWRDHVQTYT.

Annotated Terms

The following terms have been associated with this polypeptide:

Vocabulary: **Biological Process**
Term	Definition
GO:0030163	protein catabolic process
GO:0007005	mitochondrion organization
GO:0034599	cellular response to oxidative stress
GO:0051131	chaperone-mediated protein complex assembly
GO:0006515	protein quality control for misfolded or incompletely synthesized proteins
GO:0070407	oxidation-dependent protein catabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR027065	Lon_Prtase
IPR003111	Lon_substr-bd

Vocabulary: **Molecular Function**
Term	Definition
GO:0003697	single-stranded DNA binding
GO:0004176	ATP-dependent peptidase activity
GO:0043565	sequence-specific DNA binding
GO:0004252	serine-type endopeptidase activity
GO:0005524	ATP binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005759	mitochondrial matrix

Orthology

View orthology data for BolC9t58100H-R-P